The demonstration that a regulatory protein linked to stress responses in plants also serves as a master switch for anti-pathogen immunity could help breeders develop more pest-resistant and climate-resilient crops.

The KAUST-led discovery suggests that, rather than focusing on individual immune signals involved in plant defenses, agricultural scientists looking to implement sustainable crop protection strategies could simply focus their efforts on this one all-important protein.

“The identification of OXI1 as a single molecular switch of immunity offers a number of big advantages in molecular breeding,” says study lead Heribert Hirt, a professor of plant science at KAUST.

Bacteria plucked from a desert plant could help crops survive heatwaves and protect the future of food
Global warming has increased the number of severe heatwaves that wreak havoc on agriculture, reduce crop yields and threaten food supplies. However, not all plants perish in extreme heat. Some have natural heat tolerance, while others acquire heat tolerance after previous exposure to higher temperatures than normal, similar to how vaccines trigger the immune system with a tiny dose of virus.

But breeding heat tolerant crops is laborious and expensive, and slightly warming entire fields is even trickier.

The human gut microbiome is a complex system of gazillions of bacteria, fungi, viruses, protists and archaea that has an enormous effect on our metabolism, health and well‐being. The same holds true for the plant rhizosphere, the crucial parts below ground: roots are immersed in a soil microbiome that provides plants with important nutrients, protects them from disease and pathogens and helps plants to adapt to environmental changes . And, similar to faecal transplants in humans, soil transplants can have a drastic effect on plant health and growth.

Bacteria isolated from the Saudi desert have demonstrated plant-growth-promoting properties that could make them useful as biofertilizers.
“The vast majority of deserts, especially in Saudi Arabia, have never been explored for agricultural potential,” says doctoral student, Abdul Aziz Eida, of KAUST’s Desert Agriculture Initiative. “Many people think deserts are sterile and inhospitable to any form of life. But there are many plants able to grow and survive in the harsh conditions found there. We believe that one of the key factors enabling plants to survive in such environments is their association with microbes in the soil.”

A new actor in the immune system of plants has been identified. KAUST scientists have identified the protein MAP4K4 is needed to mount proper defenses against environmental pathogens.
The discovery helps explain the tight control of immune signaling in plants and reveals targets in a molecular pathway that could be manipulated by crop breeders. “Our findings are directly applicable to make plants more resistant to pathogens,” says study author, Heribert Hirt, professor of plant science at KAUST’s Center for Desert Agriculture….

KAUST Discovery Issue 6, August 2018 page 7

To feed the world, food production has to be increased by about 50 percent until the year 2050. Although European and American countries can reach these aims by technological advances, many countries in dry and hot zones already chronically lack the production of enough food for their own populations and depend on imports and foreign aid. Considering that harvest losses by drought, salt and heat stresses amount to approximately 60 percent of total productivity, improvement of abiotic stress tolerance is the main aim in crop improvement in the world. Since plants have colonized land, they have evolved mechanisms to respond to changing environmental conditions and settle in extreme habitats.

As a son of an engineer who traveled widely during his career, Heribert Hirt began his life in the exotic country of Iran, before receiving his high-school education in Germany and then studying biochemistry at the University of Cape Town and then later at the University of Vienna, from where he received his PhD in 1987. He then worked as a postdoctoral fellow in Vienna, Oxford, and Wageningen, before starting his own group at the University of Vienna in 1993. It was also in Vienna that he became professor of genetics in 1997, followed by vice-director of the Gregor Mendel Institute of Plant Molecular Biology, and later head of the Plant Molecular Biology Department of the University of Vienna. In 2007, he decided that it was time for new challenges and accepted an appointment in France to direct the Paris-based INRA-CNRS Plant Genomics Institute for the following 7 years. In 2014, Heribert embarked on yet another challenge by accepting the role to head up the Center for Desert Agriculture at King Abdullah University of Sciences and Technology (KAUST) in Saudi Arabia.

Seed Fund spring 2014 winners pose with members of the Seed Fund team during the Spring Gala 2014.

The winning teams’ project titles and members were: “ZAD – Microbes to improve agriculture under drought, heat and salinity” (Prof. Heribert Hirt, Biological Sciences and Engineering Division (BESE); Prof. Daniele Daffonchio, BESE; Dr. Axel de Zelicourt, postdoctoral fellow, Center for Desert Agriculture (CDA); Dr. Feras F. Lafi, Research Scientist, CDA; Dr. Ramona Marasco, postdoctoral fellow, Red Sea Research Center)…

Today, Saudi Arabia becomes the first country in the GCC to observe Fascination of Plants Day (FOPD), due to the efforts of the KAUST Center for Desert Agriculture. This global event is an effort led by the European Plant Science Organization (EPSO) to raise awareness about plants and plant science. In its third year, over 50 countries will observe FOPD, 26 of which are outside of continental Europe. “The importance of plants for providing food and feed is essential to every country in the world. However, a particularity of the Middle East is its extreme arid and hot climate conditions, which are major limiting factors for agricultural production,” said Heribert Hirt, professor of bioscience and director of KAUST’s Center for Desert Agriculture (CDA). Hirt is the former president of the EPSO, which gives him a unique perspective on bringing FOPD to the Middle East. “The EPSO started Fascination of Plants Day to raise awareness about the importance of plants in all facets of human life. In fact it’s the first and only world initiative that focuses on the importance of plants for human life,” said Hirt….

“The world’s population stands at 6.5 billion people right now. By the year 2050, we will be 9.5 billion. So we will have a gap of about 3 billion people to feed in the next 35 years,” said Prof. Heribert Hirt, Director of KAUST’s Center for Desert Agriculture. An upcoming international conference at KAUST, titled Desert Rhizosphere Microbes for Future Sustainable Agriculture, to be held on November 3-5, 2014, will focus on examining ways to help plants and crops survive and thrive in extreme environmental conditions…..

Professor Heribert Hirt, formerly Associate Director of the Center for Desert Agriculture, has been appointed Director of the center. Professor Mark Tester has taken over the role of Associate Director with his responsibilities focusing on desert agriculture and translational research…

Four KAUST members were recently noted on the highly select “Thomson Reuters Highly Cited Researchers 2014.” Congratulations to Jean M.J. Frechet (Chemistry), Victor M. Calo (Computer Science), Mohamed Eddaoudi (Chemistry), and Heribert Hirt (Plant & Animal Science). Highly Cited Researchers 2014 represents some of world’s leading scientific minds. Over three thousand researchers earned the distinction by writing the greatest numbers of reports officially designated by Essential Science Indicators℠ as Highly Cited Papers—ranking among the top 1% most cited for their subject field and year of publication, earning them the mark of exceptional impact….

• International Symposium Microbe – Assisted Crop Production miCROPe, (December 4 to 7, 2017) Vienna, Austria http://www.micrope.org/
• The 5th Arab-American Frontiers Symposium (November 2 to 4, 2017) Rabat, Morocco http://sites.nationalacademies.org/PGA/dsc/AAFrontiers/PGA_178215

• The RhizoSphere4 Conference (June 21 to 25, 2015) Maastricht, Netherlandshttps://globalsoilbiodiversity.org/node/462

• 10th International PGPR workshop (June 16 to 19, 2015) Liege, Belgiumhttp://www.events.gembloux.ulg.ac.be/pgpr2015/program/

• “Desert Rhizosphere Microbes for Future Sustainable SMART Agriculture” Saad MM., oral presentation, at International Symposium Microbe -Assisted Crop Production miCROPe (December 4 to 7, 2017) Vienna, Austria

• “Desert Rhizosphere Microbes for Future Sustainable SMART Agriculture” Saad MM., oral presentation, at The 5nd Arab-American Frontiers Symposium (November 2 to 4, 2017) Rabat, Morocco
• “Desert Microbes for Future Sustainable Agriculture in Arid Region” Saad MM., oral presentation at 1st International conference on applied microbiology its applications, (March 1 to 3, 2016) Giza, Egypt
• “ZAD-KAUST: Increase crop tolerance to biotic and abiotic stresses” Saad MM., oral presentation at Global forum for Innovations in Agriculture (GFIA 2016), (February 16 to 17, 2016) Abu Dhabi, United Arab Emirates
• “DARWIN 21: A global knowledge base of desert rhizosphere microbes and their roles in plant stress tolerance” Saad MM., oral presentation, at The RhizoSphere4 Conference (June 21 to 25, 2015) Maastricht, Netherlands
• “Desert Root endophytes enhance the growth of Arabidopsis thaliana under stress conditions” Saad MM., oral presentation, at 10th International PGPR workshop (June 16 to 19, 2015) Liege, Belgium
• “DARWIN 21: A global knowledge base of desert rhizosphere microbes and their roles in plant stress tolerance” Saad MM., oral presentation, at The 2nd Arab-American Frontiers Symposium (December 13 to 15, 2014) Muscat, Oman

Patent: H Hirt, A de Zelicourt, M Saad – US Patent App. 16/048,142, 2018
Compositions and methods for increasing salt tolerance in plants
Inventors: Heribert Hirt, Axel de Zelicourt, Maged Saad
Publication date: 2018/11/29
Patent office: US
Application number: 16048142

Description: Methods and compositions for increasing tolerance to abiotic stresses in plants are provided. One embodiment provides a method for increasing salt tolerance in a plant by treating the plant with an amount of 2-keto-4-methylthiobutyric acid (KMBA) effective to increase salt tolerance in the plant relative to an untreated plant. Typically KMBA is in an aqueous solution used to irrigate the plant, seed, or seedling. In a preferred embodiment, the aqueous solution is a salt solution, including but not limited to seawater. The KMBA is typically present in the aqueous solution at 1.0 to 250 nM, preferably at least 100 nM.

Scholar articles: https://patents.google.com/patent/US20180338493A1/en
Compositions and methods for increasing salt tolerance in plants

The fourth “Fascination of Plants Day 2017” will be launched under the umbrella of the European Plant Science Organisation (EPSO). The goal of this activity is to get as many people as possible around the world fascinated by plants and enthused about the importance of plant science for agriculture, in sustainably producing food, as well as for horticulture, forestry, and all of the non-food products such as paper, timber, chemicals, energy, and pharmaceuticals. The role of plants in environmental conservation will also be a key message….

Since agriculture stands as a major pillar in the Bill & Melinda Gates Foundation’s work in improving the lives of women, children and communities in the developing world, Mr. Gates took part in a roundtable discussion with KAUST faculty on the topic of agriculture research in arid and harsh conditions. The group focused on ideas and opportunities to align KAUST’s research pursuits with the Gates Foundation. Following the discussion, Mr. Gates had the opportunity to visit several KAUST laboratories and observed first-hand the innovative water-efficient and sustainable systems of food production being developed….

“The world’s population stands at 6.5 billion people right now. By the year 2050, we will be 9.5 billion. So we will have a gap of about 3 billion people to feed in the next 35 years,” said Prof. Heribert Hirt, Director of KAUST’s Center for Desert Agriculture. An upcoming international conference at KAUST, titled Desert Rhizosphere Microbes for Future Sustainable Agriculture, to be held on November 3-5, 2014, will focus on examining ways to help plants and crops survive and thrive in extreme environmental conditions…